CLIG Agar Medium

Cosmo Bio LTD

Catalog No: KYO-03401

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Purpose of Use

For screening Enterohemorrhagic Escherichia coli O157:H7/-
CLIG Agar (cellobiose added LIG agar medium)

Characteristics

CLIG Agar enables estimation and confirmation of E. coli by confirmation of acid
production from lactose and cellobiose, indole and -glucuronidase production, and is
useful for screening for enterohemorrhagic Escherichia coli O157:H7/-.

 Composition (1 L = 41.0 g)

• Casein peptone 7.5 g MUG 0.02 g
• Meat peptone 2.5 g Sodium chloride 5.0 g
• Lactose 1.0 g Phenol red 0.025 g
• Cellobiose 10.0 g Agar powder 14.9 g
• Tryptophan 0.1 g
• Purified water 1,000 ml pH 7.4s

Preparation method

41.0 g of this product is added to 1,000 mL of purified water, and dissolved by heating.
2.5 to 3.0 mL of this agar medium is poured into each small size of test tube, and
high-pressure sterilization is performed at 115 degrees for 15 minutes.
After sterilization, solidify it as semi-slant medium such as TSI agar medium etc.

Test method

Inoculate the test bacterial strains in the bottom part and on the slant part, and
incubate at 35 to 37 degrees for 18 to 24 hours.
Caution: Do not keep it with a tight stopper (cap) during culture.

Interpretation of results

	Enterohemorrhagic E. coli O157:H7/-	Other E. coli
Bottom part	Change to yellow	Change to yellow
Slant part	Change to red	Change to red
Cytochrome oxidase	Negative	Negative
Indole production ^*1)	Positive	Positive
Blue fluorescence under UV irradiation ^*2)	Negative	Positive

 ^*1)Testing by indole spot test ^*2) Ultraviolet at 365 nm

After confirming the above properties, conduct agglutination test using anti-O157
serum.